Generator

Part:BBa_K318013:Design

Designed by: Nathaniel Pantalone, Justin Vrana   Group: iGEM10_Wisconsin-Madison   (2010-07-13)


T + pLac + RBS + Int (P22) + T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 545
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To minimize potential transcription due to upstream promoter activity, a terminator was placed upstream of the pLac promoter.  A terminator was placed downstream of the Int encoding sequence to end transcription after the recombinase is produced.  Note that to fully repress the pLac promoter, overproduction of the lacI repressor protein is necessary.


Source

Biobricks

References