![](https://parts.igem.org/images/partbypart/icon_generator.png)
Generator
Part:BBa_K318013:Design
Designed by: Nathaniel Pantalone, Justin Vrana Group: iGEM10_Wisconsin-Madison (2010-07-13)
T + pLac + RBS + Int (P22) + T
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 545
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
To minimize potential transcription due to upstream promoter activity, a terminator was placed upstream of the pLac promoter. A terminator was placed downstream of the Int encoding sequence to end transcription after the recombinase is produced. Note that to fully repress the pLac promoter, overproduction of the lacI repressor protein is necessary.
Source
Biobricks